complementary DNA

A study of complementary DNA cloning and sequence determination of rabbit type PLA2 with potent bactericidal activity

具强杀菌活性的兔Ⅱ型磷脂酶A2互补DNA克隆及其顺序确定的研究

DNA template directs organic synthesis by sequence-specificity recruiting reactants linked to complementary DNA .

DNA模板指导的有机合成反应具有序列特异性特点。

Methods : ( 1 ) Reverse transcription-polymerase chain reaction ( RT-PCR ) was use to obtain the TR gene complementary DNA ( cDNA ) .

方法：（1）进行逆转录-聚合酶链式反应（RTPCR），获得TR基因cDNA；

Recently we have prepared large amount of purified pEH 920 DNA which is inserted by complementary DNA of dengue virus 2 RNA .

新近制备了大量纯化的pEH920DNA，该质粒DNA插入了登革病毒2型核酸片段的互补DNA。

Cyclic voltammetry was used to characterize the ssDNA immobilization and hybridization with its complementary DNA sequences by using methylene blue as indicator .

在硬脂酸铝离子膜上进行DNA探针的固定和与目标基因的杂交。

Fungus-transmitted wheat mosaic virus ( FWMV-C ) isolated from Henan , China was used for viral RNA extraction and complementary DNA synthesis .

以采自河南的真菌传小麦花叶病毒（FWMV－C）为材料，抽提病毒RNA，合成互补DNA（CDNA）。

Molecular beacons are novel fluorescence oligonucleotide probes fluorescing upon hybridization to their complementary DNA / RNA targets with excellent sensitivity and high selectivity .

分子信标是一种高灵敏度、高特异性的新型荧光核酸探针。它在与互补DNA/RNA靶序列杂交时放出荧光。

The chicken growth hormone ( GH ) complementary DNA ( cDNA ) have been isolated from a cDNA library , which was constructed with meat-type chicken breed pituitary .

本研究从肉用品种鸡的垂体文库中克隆分离了鸡生长激素cDNA，并作了核苷酸序列测定。

The complementary DNA ( cDNA ) of prolactin ( PRL ) was cloned and sequenced ( GenBank accession number : AM180924 ) .

首次采用RT-PCR技术扩增、克隆了番鸭催乳素基因，获得了全长为765bp的cDNA序列（基因登陆号：AM180924）。

Therefore , we tried to clone the complementary DNA of hBMP-3 gene by RT-PCR , for the purpose of producing human morphogenetic protein in the mammary glands of transgenic animals .

鉴于此，本研究拟通过RT-PCR的方法克隆该基因，为利用转基因动物乳腺生产人骨形成蛋白创造条件。

It is possible to make specific complementary DNA 's （ cDNA 's ） that can serve as molecular probes to seek out the messenger RNA 's （ mRNA 's ） of the peptide hormones .

科学家可以制造出特异的（specific）互补DNA's（cDNA's），以此作为分子探子（molecularprobe），探觅出肽激素的信使RNA's（mRNA's）。

In the absence of target DNA , complementary DNA showed the status of the single-stranded , RCA reaction was initiated by addition of the circular template , polymerase , ligase and dNTP .

在没有目标物DNA时，互补DNA呈现单链状态，其在环状模板，聚合酶，连接酶以及dNTP的存在下进行RCA扩增。

The general strategy for cloning chymosin gene is to synthesize complementary DNA ( cDNA ) by reverse transcription of mRNA fron which introns have been spliced out and then the cDNA is cloned and expressed in a suitable host .

克隆凝乳酶的一般策略是经过mRNA反转录合成cDNA，将内涵子拼接掉后把cDNA克隆在表达宿主中进行表达。

It binds to complementary RNA or DNA with specificity and form a triplex complex with highly stability .

它与DNA或RNA杂交特异性很强，可与靶基因形成稳定的三螺旋结构。

The hybridization sensitivity of quartz crystal DNA biosensor is 10ng / ml. In the range of 5 ~ 40ng / ml , there was a linear correlation between responding signals and concentrations of complementary single strand DNA with a high specificity .

结果显示，10MHz石英晶体DNA传感器的杂交灵敏度为10ng/ml，在5～40ng/ml的浓度范围内，响应信号与检测浓度之间具有良好的线性相关性，其特异性也比较高。

Methods Bis PNA probe for detecting HBV was immobilized on the surface of gene sensor array . Different concentrations of the " RecA protein complementary single strand DNA " probes were added after the bis PNA probes were hybridized with corresponding target DNA .

方法基因传感器阵列固定上针对乙肝病毒的bisPNA探针，加入靶DNA与之反应，然后加入预先处理过的不同浓度（0.5～6mg/ml）的RecA蛋白互补单链DNA探针。

Primary Study on Interaction Change of Complementary Base Pairs in DNA during H Bond Breaking ( Continued )

DNA分子互补碱基对氢键解裂前后相互作用变化的初步研究（续）

Changes of nuclear RNA and polysomal RNA complementary to the non-repeated DNA sequences in rat hepatoma

与单一顺序DNA互补的大鼠肝癌核RNA和聚核糖核蛋白体RNA的变化

By measuring the amplification of RLS signal , complete complementary and bases mismatched DNA sequences can easily be detected and identified .

通过测定放大的RLS信号，完全互补和有碱基错配的DNA序列能很容易地被检测和识别。

The aptamer probe has a electroactive ferrocene-labeled tail sequence that is complementary to the surface-tethered DNA strands with a predesigned low melting temperature .

该适体探针尾端修饰电活性基团二茂铁，且单个尾端序列与表面固定的短链寡核苷酸探针的熔链温度较低。